772800+ entries in 0.528s
Bugpowder: mircea_popescu:
That was made by
the former
Bugpowder: Then you just sort millions of cells over and over again
till you enrich
the color you want.
mircea_popescu: Bugpowder did you obtain
the painting by smear selected bacteria or by smearing uniform bacteria with particular mutagen ?
Bugpowder: OR you can insert
the gene into
the hyper somatic recombination region of B cells (this is how antibody diversity is generated in your own immune system), and
then let
the B cells mutate
the protein for you
Bugpowder: but a few work... Just need
to find
them.. by flipping 2-3 letters at a
time
Bugpowder: Most mutations kill
the fluorescence (they don't match literate word, as a metaphor)
Bugpowder: Here is a painting of various color fluoresncet bacteria on a plate... created by
this game...
Bugpowder: This is how one gene, GFP,
the green fluorescent protein
Bugpowder: and you can put
them under a fluorescent light
to see if you A) fucked
the protein up or B) changed its color slightly.
Take your best hits, sequence
the gene
to see what you've got,
then repeat
the process.
Bugpowder: each bacteria
that got one of
the mutant genes in it will grow an independent colony
Bugpowder: then you mix
the genes into a bunch of bacteria, and dilute
the solution really far down,
then spread it over many agar plates
Bugpowder: your new gene sequence now has randomizations at
those spots, as specified by your primer blend
Bugpowder: So you do a PCR reaction (make billions of copies of
the DNA), but use primers
that have
these selective random patches at
the points in
the sequence you want
to sample
the variation space in
Bugpowder: you suspect
that a few amino acids near
the core chromophore will influence
the color
Bugpowder: Say you want
to evolve a fluorescent protein
to glow a different color
Bugpowder: OR you can specify a blend of
two,
three or all four randomly assorted at
that position
Bugpowder: though some represent
the same letter
Bugpowder: since you need 3
to actually code an amino acid.
Bugpowder: trying
to find an new one
that matches
Bugpowder: You use degenerate PCR primers
to evolve and screen fluorescent protein genes
Bugpowder: Well, it's not exactly
the same game but similar enough
Bugpowder: mircea_popescu: I played
that game quite a bit in grad school.
mike_c: hah, yeah, i read
that. but
that is a minor consideration against
the order of magnitude changes when it goes up.
benkay: for failing
to capitalize on high price.
benkay: mike_c:
the basic gist is
that you can't spend it when btc price is going up 'cause derp, but
then when you don't spend it in
time you get a reaming from investors.
mike_c: benkay: recently? don't
think so. i've been around for a couple discussions on
the
topic.
benkay: mike_c: did you see mircea_popescu's point a few days ago about
the flipside of "you can raise it but you can't spend it"?
mircea_popescu: drama chick after performance : "how come people on stage never
throw
tomatoes @
the audience"
kakobrekla: well since you are already
taking complaints, in opera next witch already uses webkit, some charts get broked
benkay: and i
think it's a blocking call.
mike_c: yes,
that one i didn't want
to bother serving locally. but i will probably change
that b/c
their server is down more
than mine.
benkay: that'd make you a better dev
than most webbers.
mike_c: thanks moiety.
there is more
to come on
that page.
moiety: i was just reading
the income page - nice page mike_c
mike_c: you don't have
to clear cache. i version my static files like a good webdev.
benkay: highchart js is
the long-loading one.
benkay: no man fuckit! debug in -assets!
this is how we roll.
benkay: yeah it may be a browser
thing
benkay goes back
to
the ragel documentation
benkay: i
think
the assettes crashed your server, mike_c.
mike_c: Apocalyptic:
thanks. also, i believe awhile ago you mentioned you were interested in
the income investments section, so check out
http://www.btcalpha.com/income/. it's version 1, but still some useful info i
think.
mike_c: thanks. credit
to kakobrekla for sending
that along. very cool of him.
mike_c: August
through january. i
think
that's 5
mircea_popescu: "hey guise! check out
this neat
thing i did" "x" "o fuck me".
benkay: talking about
the scoop, mike_c :0
ozbot: Panacea Opens
The Books - Btc Alpha
mircea_popescu: aka
the "We must spy because you're sueing us about our spying" defense.
mircea_popescu: "A number of government lawyers involved in lawsuits over
the NSA phone-records program believe federal-court rules on preserving evidence related
to lawsuits require
the agency
to stop routinely destroying older phone records"
mike_c: 1) stop withdrawals. 2) use your 'unlimited account'
to buy btc at 260. 3) use whatever you have left in
the sofa cushions
to process some withdrawals and restore some confidence 4) sell some of your cheap coins and use funds
to process more withdrawals. 5) goto step 4.
benkay: at least in
their minds...
benkay: not
that good of a joke
moiety: this is how gox is protecting itself from closure. If
they don't let anyone withdraw ever again,
they can't ever really close
bloctoc: wondering if
that's where mtgox needs
the price
to be
to recapitalize.
bloctoc: if you look at 24 hour chart,
there seems
to be a willingness
to buy at 261
Duffer1: did
they suspend
trading or did
the market stop selling?
gribble: MtGox BTCUSD
ticker | Best bid: 262.345, Best ask: 264.695, Bid-ask spread: 2.35000, Last
trade: 262.345, 24 hour volume: 33931.23630368, 24 hour low: 257.10001, 24 hour high: 298.0, 24 hour vwap: 265.3911
bloctoc: I apologize if it's already been discussed, but does anyone seen significance in mtgox stopping
the drop
today?
moiety: lol i had a parrot
that meowed and did
the phone
benkay: my heart sinks when
truffles comes in.
jayk: especially ones
that sound exactly like mine
jayk: i really hate commercials
that have a doorbell
moiety: how many bips are
there?
mircea_popescu: the argument isn't "o wow look btc is broken",
the argument merely is "o hey, remember when gavin was a
total asshat in 2012 ?"
mircea_popescu: asciilifeform ono. it pulls up something from
the stack.
mircea_popescu: just establishing your credentials
to bitch at
them >D
benkay: don't
they know
the assettes only get off on public nudity?
moiety: lol i would love it if
they did
moiety: i love how
they went on location as probably
too embarrassed
to strip pff anywhere else
moiety: i still cant figure out
the btcbeauties project apart from
trying
to hide
the fat one
mircea_popescu: asciilifeform look into bip-12
too while you're at it.
mircea_popescu: ThickAsThieves
this is a sore spot power rangers would rather not go into.
mircea_popescu: in his defense
they "updated"
the site like 5x in
the interval, because you defo need moar logos and shit
ThickAsThieves: are bitcoin software updates even going
to be possible for much longer?
benkay: what was
this forced voting
thong?
moiety: went looking for
that asciilifeform lol